I just saw

; Tools
 
M563 P0 S"Mosquito" D0 H1 F0:1                     ; define tool 0

I try to remove :1

It worked.... thanks anyway.

I just saw

; Tools
 
M563 P0 S"Mosquito" D0 H1 F0:1                     ; define tool 0

I try to remove :1

It worked.... thanks anyway.

and here's the beginning of the gcode

Edit: I think line 27 M107 overrides the parameter of the config and turns the fan off, even if it's over 45°C...

;FLAVOR:RepRap
;TIME:4567
;Filament used: 3.78854m
;Layer height: 0.2
;MINX:81.328
;MINY:89.102
;MINZ:0.2
;MAXX:144.791
;MAXY:120.899
;MAXZ:48
;Generated with Cura_SteamEngine 4.8.0
T0
M190 S50
M104 S210
M109 S210
M82 ;absolute extrusion mode
G28 ;Home
G1 Z15.0 F6000 ;Move the platform down 15mm
;Prime the extruder
G92 E0
G1 F200 E10
G92 E0
M83 ;relative extrusion mode
G1 F1500 E-6.5
;LAYER_COUNT:240
;LAYER:0
M107
G0 F3600 X84.693 Y92.804 Z0.2
;TYPE:SKIRT
G1 F1500 E6.5
G1 X85.204 Y92.414 E0.02138
G1 X85.578 Y92.152 E0.01519
G1 X86.247 Y91.732 E0.02627
G1 X86.954 Y91.382 E0.02624
G1 X87.6 Y91.136 E0.02299
G1 X88.092 Y90.973 E0.01724
G1 X88.853 Y90.763 E0.02626
G1 X89.813 Y90.611 E0.03233
G1 X90.454 Y90.555 E0.0214
G1 X113.599 Y89.149 E0.77122
G1 X114.222 Y89.118 E0.02075
G1 X114.534 Y89.108 E0.01038
G1 X115.146 Y89.102 E0.02036
G1 X115.456 Y89.106 E0.01031
G1 X116.056 Y89.124 E0.01997
G1 X116.375 Y89.14 E0.01062
G1 X116.952 Y89.181 E0.01924
G1 X117.256 Y89.208 E0.01015
G1 X117.817 Y89.27 E0.01877
G1 X118.114 Y89.309 E0.00996
G1 X118.669 Y89.392 E0.01866
G1 X118.949 Y89.439 E0.00944
G1 X119.505 Y89.543 E0.01881
G1 X119.789 Y89.602 E0.00965
G1 X120.338 Y89.726 E0.01872
G1 X120.638 Y89.8 E0.01028
G1 X121.183 Y89.946 E0.01877
G1 X121.446 Y90.021 E0.0091
G1 X121.966 Y90.18 E0.01809
G1 X122.253 Y90.274 E0.01004
G1 X122.755 Y90.449 E0.01768
G1 X122.999 Y90.539 E0.00865
G1 X123.488 Y90.728 E0.01744
G1 X123.743 Y90.831 E0.00915
G1 X124.22 Y91.035 E0.01726
G1 X124.476 Y91.15 E0.00933
G1 X124.941 Y91.369 E0.0171
G1 X125.18 Y91.487 E0.00887
G1 X125.632 Y91.719 E0.0169
G1 X125.868 Y91.845 E0.0089
G1 X126.338 Y92.107 E0.0179
G1 X126.606 Y92.264 E0.01033
G1 X127.084 Y92.556 E0.01863
G1 X127.29 Y92.686 E0.0081
G1 X127.733 Y92.976 E0.01761
G1 X127.999 Y93.158 E0.01072
G1 X128.417 Y93.457 E0.01709
G1 X128.605 Y93.595 E0.00776
G1 X129.083 Y93.959 E0.01998
G1 X129.361 Y94.182 E0.01185
G1 X129.752 Y94.509 E0.01695
G1 X129.935 Y94.667 E0.00804
G1 X130.382 Y95.065 E0.01991
G1 X130.632 Y95.297 E0.01134
G1 X131.062 Y95.715 E0.01995
G1 X131.276 Y95.932 E0.01014
G1 X131.628 Y96.302 E0.01699
G1 X131.813 Y96.503 E0.00909
G1 X132.275 Y97.024 E0.02316
G1 X132.492 Y97.28 E0.01116
G1 X132.826 Y97.691 E0.01761
G1 X132.994 Y97.905 E0.00905
G1 X133.298 Y98.307 E0.01676
G1 X133.46 Y98.53 E0.00917
G1 X133.75 Y98.943 E0.01678
G1 X133.889 Y99.146 E0.00818
G1 X134.179 Y99.588 E0.01758
G1 X134.353 Y99.867 E0.01094
G1 X134.617 Y100.309 E0.01712
G1 X134.988 Y101.007 E0.02629
G1 X135.31 Y101.774 E0.02767
G1 X135.57 Y102.519 E0.02624
G1 X135.753 Y103.288 E0.02629
G1 X135.86 Y104.111 E0.0276
G1 X135.891 Y104.515 E0.01348
G1 X135.912 Y105.305 E0.02628
G1 X135.852 Y106.092 E0.02625
G1 X135.657 Y107.098 E0.03408
G1 X135.543 Y107.531 E0.01489
G1 X135.304 Y108.283 E0.02624
G1 X134.989 Y109.008 E0.02629
G1 X134.745 Y109.463 E0.01717
G1 X134.422 Y110.019 E0.02139
G1 X134.23 Y110.332 E0.01221
G1 X133.944 Y110.775 E0.01754
G1 X133.777 Y111.022 E0.00992
G1 X133.486 Y111.437 E0.01686
G1 X133.323 Y111.661 E0.00921
G1 X133.02 Y112.062 E0.01672
G1 X132.865 Y112.261 E0.00839
G1 X132.55 Y112.651 E0.01667
G1 X132.372 Y112.864 E0.00923
G1 X132.036 Y113.251 E0.01705
G1 X131.871 Y113.434 E0.0082
G1 X131.475 Y113.862 E0.01939
G1 X131.25 Y114.096 E0.0108
G1 X130.886 Y114.458 E0.01707
G1 X130.699 Y114.637 E0.00861
G1 X130.325 Y114.985 E0.01699
G1 X130.131 Y115.16 E0.00869
G1 X129.678 Y115.554 E0.01997
G1 X129.435 Y115.757 E0.01053
G1 X129.038 Y116.075 E0.01692
G1 X128.821 Y116.243 E0.00913
G1 X128.339 Y116.602 E0.01999
G1 X128.062 Y116.799 E0.01131
G1 X127.635 Y117.089 E0.01717
G1 X127.451 Y117.21 E0.00732
G1 X126.989 Y117.505 E0.01823
G1 X126.71 Y117.675 E0.01087
G1 X126.223 Y117.958 E0.01873
G1 X125.995 Y118.086 E0.0087
G1 X125.55 Y118.325 E0.0168
G1 X125.298 Y118.454 E0.00942
G1 X124.787 Y118.705 E0.01894
G1 X124.486 Y118.845 E0.01104
G1 X124.015 Y119.052 E0.01711
G1 X123.807 Y119.14 E0.00751
G1 X123.252 Y119.365 E0.01992
G1 X122.962 Y119.476 E0.01033
G1 X122.456 Y119.658 E0.01789
G1 X122.171 Y119.754 E0.01
G1 X121.669 Y119.913 E0.01751
G1 X121.398 Y119.993 E0.0094
G1 X120.875 Y120.138 E0.01805
G1 X120.6 Y120.209 E0.00945
G1 X120.063 Y120.337 E0.01836
G1 X119.828 Y120.39 E0.00801
G1 X119.328 Y120.493 E0.01698
G1 X119.021 Y120.549 E0.01038
G1 X118.523 Y120.631 E0.01679
G1 X118.268 Y120.669 E0.00857
G1 X117.771 Y120.734 E0.01667
G1 X117.537 Y120.761 E0.00783
G1 X117.038 Y120.811 E0.01668
G1 X116.762 Y120.834 E0.00921
G1 X116.188 Y120.871 E0.01913
G1 X115.874 Y120.885 E0.01045
G1 X115.336 Y120.898 E0.0179
G1 X115.025 Y120.899 E0.01034
G1 X114.518 Y120.891 E0.01686
G1 X114.292 Y120.884 E0.00752
G1 X113.672 Y120.856 E0.02064
G1 X112.782 Y120.804 E0.02965
G1 X111.717 Y120.737 E0.03549
G1 X110.258 Y120.649 E0.04861
G1 X109.705 Y120.614 E0.01843
G1 X108.365 Y120.534 E0.04465
G1 X107.543 Y120.483 E0.02739
G1 X106.918 Y120.446 E0.02082
G1 X106.009 Y120.389 E0.03029
G1 X105.486 Y120.359 E0.01742
G1 X102.82 Y120.196 E0.08884
G1 X101.671 Y120.129 E0.03828
G1 X97.724 Y119.888 E0.13152
G1 X96.939 Y119.842 E0.02615
G1 X96.13 Y119.791 E0.02696
G1 X90.249 Y119.432 E0.19597
G1 X89.464 Y119.344 E0.02627
G1 X89.004 Y119.255 E0.015

Here's the config

; Configuration file for Duet WiFi (firmware version 2.00)
; executed by the firmware on start-up
;
; generated by RepRapFirmware Configuration Tool v3.2.3 on Sat Feb 27 2021 02:41:58 GMT+0100 (Central European Standard Time)

; General preferences
G90                                                ; send absolute coordinates...
M83                                                ; ...but relative extruder moves
M550 P"Ender 5"                                    ; set printer name

; Network
M552 S1                                            ; enable network
M586 P0 S1                                         ; enable HTTP
M586 P1 S0                                         ; disable FTP
M586 P2 S0                                         ; disable Telnet

; Drives
M569 P0 S0                                         ; physical drive 0 goes backwards
M569 P1 S1                                         ; physical drive 1 goes forwards
M569 P2 S0                                         ; physical drive 2 goes backwards
M569 P3 S1                                         ; physical drive 3 goes forwards
M584 X0 Y1 Z2 E3                                   ; set drive mapping
M350 X16 Y16 Z16 E16 I1                            ; configure microstepping with interpolation
M92 X80.00 Y80.00 Z800.00 E404.28                  ; set steps per mm
M566 X1200.00 Y1200.00 Z60.00 E300.00              ; set maximum instantaneous speed changes (mm/min)
M203 X30000.00 Y30000.00 Z300.00 E6000.00          ; set maximum speeds (mm/min)
M201 X500.00 Y500.00 Z20.00 E5000.00               ; set accelerations (mm/s^2)
M906 X800 Y800 Z800 E1000 I30                      ; set motor currents (mA) and motor idle factor in per cent
M84 S30                                            ; Set idle timeout

; Axis Limits
M208 X0 Y0 Z0 S1                                   ; set axis minima
M208 X230 Y210 Z300 S0                             ; set axis maxima

; Endstops
M574 X1 Y1 Z1 S1                                   ; set active high endstops

; Z-Probe
M558 P0 H1 F0 T3000                                ; disable Z probe but set dive height, probe speed and travel speed
M557 X20:200 Y20:200 S50                           ; define mesh grid

; Heaters
M307 H0 B0 S1.00                                   ; disable bang-bang mode for the bed heater and set PWM limit
M305 P0 T100000 B4092 R4700                        ; set thermistor + ADC parameters for heater 0
M143 H0 S150                                       ; set temperature limit for heater 0 to 150C
M305 P1 T500000 B4723 C1.196220e-7 R4700           ; set thermistor + ADC parameters for heater 1
M143 H1 S275                                       ; set temperature limit for heater 1 to 275C

; Fans
M106 P0 S0 I0 F500 H-1                             ; set fan 0 value, PWM signal inversion and frequency. Thermostatic control is turned off
M106 P1 S1 I0 F500 H1 T45                          ; set fan 1 value, PWM signal inversion and frequency. Thermostatic control is turned on

; Tools
M563 P0 S"Mosquito" D0 H1 F0:1                     ; define tool 0
G10 P0 X0 Y0 Z0                                    ; set tool 0 axis offsets
G10 P0 R0 S0                                       ; set initial tool 0 active and standby temperatures to 0C

; Custom settings are not defined

M671 X30:30:200:200 Y15:185:185:15 P0.7            ; adjusting screws at rear right (30,15), front right (30,185), front left (200,185), rear left (200,15), thread pitch 0.7mm (for M4)



; Miscellaneous
M501                                               ; load saved parameters from non-volatile memory
M911 S21 R23 P"M913 X0 Y0 G91 M83 G1 Z3 E-5 F1000" ; set voltage thresholds and actions to run on power loss
T0                                                 ; select first tool

Hello Makers,
I realized my first Duet 2 project. All the base functions are running and are ready to print.
My latest and first unsolvable (by me) problem is that when I start Cura code my Extruder fan turns off when the printer starts the first layer. I assume that it will turn on after the first layer and that the part cooling fan and the extruder fans are connected somehow. Otherwise the extruder fan starts at 45°C as it should.

What went wrong with my setting?

PS:
RRF 2.03 / Duet 2 Wifi